Cell Line Authentication in India has a vital role in various fields of specialized study and research. Cell Line Authentication is a process by which a human cell-line identity is verified and extensively used in biomedical research. It is an important research tool, used in a variety of areas such as medicine, genetics, vaccine development, cell biology, etc., and required every time a new line is established or acquired. To name a few other applications of Cell Line Authentication in India are drug discovery, reconstructive drugs, screening, and toxicity testing.
Types of Errors Detected in a Cell Line Authentication DNA Test
There are three major types of errors or problems that can be detected using Cell Line Authentication. These are:
- Misidentification at Source – When the cell lines of interest are collected, they may be misidentified. This is one of the major causes of wastage of precious time in a study or research, where the researcher keeps on getting the wrong results.
- Cross-Contamination – Environmental pollutants like dust and fungus can easily contaminate the cells under study. To overcome this problem, maintaining a contamination-free environment in the lab or the working and at the storage areas is necessary. However, despite the care, contamination of the cells may be inevitable. A Cell Line Authentication Test can help you accurately identify such errors.
- Genetic Drift – Over time, the cells may completely lose their identity and originality, as they may become totally different cells due to genetic drift.
Method of a Cell Line Authentication Test in India
For a Cell Line Authentication DNA Test, the STR profiling method is used.. ‘STR’ stands for ‘short tandem repeats’. These are basically a few base-pair long repeating sequences on the DNA molecule and are used for the sake of simplicity and ease in the authentication process.
Here is the stepwise process of the Cell Line Authentication test:
- Collecting the DNA sample from the cell lines of interest
- Isolating the STR segments of the DNA
- Subjecting the STRs to PCR amplification
- Capillary electrophoresis
- Creating the DNA profile
DNA Forensics Laboratory – For Accurate Cell Line Authentication Tests in India
At DNA Forensics Laboratory Pvt. Ltd., we use standardized techniques which detect misidentified, genetically drifted, or cross-contaminated cells that invalidate research results. For the analysis, we use STR typing, and after getting the data, we compare it with the cell line database.
When is cell line authentication to be performed?
- At an early stage, in order to establish the cell line identity.
- Before starting of new series of experiments.
- When the behavior of the cell line is not as expected, or the results of an experiment are not reproducible.
- The culture is actively growing; hence authenticate it within two months before research publication.
Whenever in doubt about the state of your cell lines, contact us at +91 8010177771, or WhatsApp at +91 9266615552. Besides providing Cell Line Authentication in India, we also offer accredited Peace of Mind and Legal DNA Tests. At DNA Forensics Laboratory, we are the only private company providing Legal DNA Tests in India for honorable courts of Law.
